Blood Lines
by Donald Metcalf
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Contents

Preface xiii
Introduction 1
The Novel Mouse: Opening Remarks and Initial Investigations 5
Hematopoietic Subpopulations, Their Detection and Regulation 39
Analysis of Hematopoietic Tissues Using Semi-solid Cultures 57
Analyzing Semi-solid Cultures 83
Assessing Proliferative and Self-renewal Capacity and Data Presentation 107
The Importance and Design of Long-term Survival Studies 123
Histological Examination 131
Transplantation Tests 151
Diseases Involving Hematopoietic Tissues 167
Additional Analyses 211
Applying Closure 217
When is it time to stop an analysis?
What do the novel mice show?
Is hematopoiesis normal?
Publication of negative data
Is a hematological abnormality present but trivial?
How should a major hematological disease be handled?
How should an insoluble disease be responded to?
Finally, are studies on mice or mouse disease a waste of time?
References 227
AlphaMed Press is grateful to AMGEN for an unrestricted educational grant in support of the publication of Blood Lines.
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