Blood Lines
by Donald Metcalf
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Contents

Preface xiii
Introduction 1
The Novel Mouse: Opening Remarks and Initial Investigations 5
Some comments before starting
A miscellany of mouse information
The basic investigational procedures
Case history
General inspection
Initial hematological examination
Hematocrit
Platelet counts
Neonatal or fetal blood
Comment
General comments on the collection of hematopoietic tissues and preparation of cell suspensions
Collection of hematopoietic organs and autopsy
Peritoneal cells
The completed autopsy procedure
Organ weighing
Spleen
Bone marrow
Other organs
Fluorescence-Activated Cell Sorting (FACS) analyses
Sterility
Collecting organs for histological examination
The importance of cell numbers and morphology
Numbers of mice to be examined
Final comment
Hematopoietic Subpopulations, Their Detection and Regulation 39
Analysis of Hematopoietic Tissues Using Semi-solid Cultures 57
Analyzing Semi-solid Cultures 83
Assessing Proliferative and Self-renewal Capacity and Data Presentation 107
The Importance and Design of Long-term Survival Studies 123
Histological Examination 131
Transplantation Tests 151
Diseases Involving Hematopoietic Tissues 167
Additional Analyses 211
Applying Closure 217
References 227
AlphaMed Press is grateful to AMGEN for an unrestricted educational grant in support of the publication of Blood Lines.
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