Blood Lines
by Donald Metcalf
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Contents

Preface xiii
Introduction 1
The Novel Mouse: Opening Remarks and Initial Investigations 5
Hematopoietic Subpopulations, Their Detection and Regulation 39
Detection of hematopoietic precursor cells
Assays for maturing stem cells
Assays for lineage-committed progenitor cells
Regulation of hematopoietic precursors
Functional activation
Conclusions
Analysis of Hematopoietic Tissues Using Semi-solid Cultures 57
Analyzing Semi-solid Cultures 83
Assessing Proliferative and Self-renewal Capacity and Data Presentation 107
The Importance and Design of Long-term Survival Studies 123
Histological Examination 131
Transplantation Tests 151
Diseases Involving Hematopoietic Tissues 167
Additional Analyses 211
Applying Closure 217
References 227
AlphaMed Press is grateful to AMGEN for an unrestricted educational grant in support of the publication of Blood Lines.
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