Blood Lines
by Donald Metcalf
Contents
| Preface | xiii |
| Introduction | 1 |
| The Novel Mouse: Opening Remarks and Initial Investigations | 5 |
| Hematopoietic Subpopulations, Their Detection and Regulation | 39 |
| Analysis of Hematopoietic Tissues Using Semi-solid Cultures | 57 |
|
General considerations
Liquid versus semi-solid cultures Agar versus methyl cellulose cultures General technical questions The stimuli for hematopoietic colonies Inactive stimuli Additional culture techniques FACS sorted populations Colony recloning Paired daughter cell reculture Problem solving Checks for incubators |
|
| Analyzing Semi-solid Cultures | 83 |
| Assessing Proliferative and Self-renewal Capacity and Data Presentation | 107 |
| The Importance and Design of Long-term Survival Studies | 123 |
| Histological Examination | 131 |
| Transplantation Tests | 151 |
| Diseases Involving Hematopoietic Tissues | 167 |
| Additional Analyses | 211 |
| Applying Closure | 217 |
| References | 227 |
AlphaMed Press is grateful to AMGEN for an unrestricted educational grant in support of the publication of Blood Lines.
Copyright ©2007 AlphaMed Press. All Rights Reserved.